Lettuce Mosaic Virus in Lettuce Production Areas in Finike District of Antalya Province
نویسندگان
چکیده
Bu çalışmada, Antalya ili Finike ilçesi marul üretim alanlarında Marul mozayik virüsü (Lettuce mosaic virüs: LMV)’nün belirlenmesi amacıyla yürütülmüştür. Virüsün tanılama çalışmalarında DAS-ELISA (Double Antibody Sandwich Enzyme Linked Immunosorbent Assay) ve IC-RT-PCR (Immunocapture Ters Transkripsiyon Polimeraz Zincir Reaksiyonu) yöntemleri kullanılmıştır. amaçla araştırma alanından 92 örneği toplanmıştır. Örnekler LMV etmenine spesifik antibadiler kullanılarak testine tabi tutulmuştur. yöntemine göre; 5 örnek ile enfekteli bulunmuştur. testinde pozitif çıkan çalışmalarında, LMV’ye özgü primer çiftleri (1196, 1087) kullanılmış LMV’nin 800 bp’lik bir kısmı çoğaltılarak agaroz jel elektroforezde beklenen seviyede bant elde edilmiştir. çalışma ilçesinde varlığı ilk kez ortaya konulmuştur.
منابع مشابه
Characteristics of a Lettuce mosaic virus Isolate Infecting Lettuce in Korea
Lettuce mosaic virus (LMV) causes disease of plants in the family Asteraceae, especially lettuce crops. LMV isolates have previously been clustered in three main groups, LMV-Yar, LMV-Greek and LMVRoW. The first two groups, LMV-Yar and LMV-Greek, have similar characteristics such as no seed-borne transmission and non-resistance-breaking. The latter one, LMV-RoW, comprising a large percentage of ...
متن کاملLettuce Production in California
Background In the early 20 century, California produced only little lettuce . For 1909, Knott and Tavernetti [4] reported that lettuce was produced on 595 acres in California, which corresponded to 11% of the total U.S. acreage. Lettuce production increased dramatically in the 1920s (Figure 1). While 6,100 acres were harvested in 1919 , the harvested area reached 112,000 acres by 1930 . The mar...
متن کاملThe eukaryotic translation initiation factor 4E controls lettuce susceptibility to the Potyvirus Lettuce mosaic virus.
The eIF4E and eIF(iso)4E cDNAs from several genotypes of lettuce (Lactuca sativa) that are susceptible, tolerant, or resistant to infection by Lettuce mosaic virus (LMV; genus Potyvirus) were cloned and sequenced. Although Ls-eIF(iso)4E was monomorphic in sequence, three types of Ls-eIF4E differed by point sequence variations, and a short in-frame deletion in one of them. The amino acid variati...
متن کاملMolecular characterization of mo1, a recessive gene associated with Lettuce mosaic potyvirus resistance in lettuce
Lettuce mosaic, caused by the potyvirus LMV, is a serious viral disease of lettuce crops world-wide. Beside seed control, one of the leading strategies to control LMV is the use of the recessive resistance genes mo11 and mo12. In an attempt to characterize the functional role of the translation initiation factor eIF4E in lettuce susceptibility to LMV, we cloned Ls-eIF4E cDNAs from a collection ...
متن کاملVirus Diseases of Lettuce in Brazil
Several viruses have been reported to infect lettuce. The most important is Lettuce mosaic virus (LMV), a potyvirus found worldwide transmitted by seeds and aphids, in a non-persistent manner. LMV causes quite variable symptoms, including mosaic, dwarfing, failure to form proper heads, and sometimes necrotic reactions. Cultivars carrying the mo11 and mo12 genes have resistance to the common str...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Sinop Üniversitesi fen bilimleri dergisi
سال: 2023
ISSN: ['2536-4383', '2564-7873']
DOI: https://doi.org/10.33484/sinopfbd.1254988